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  Code Test Description
* Indicates send out test
  * S16005 Acetylcholine Receptor Antibody
  Specimen 1 ml serum, preferred sample
  Method Immunoprecipitation RIA
  Schedule 1–14 working days
  Indication Diagnosis of acquired Myasthenia gravis.
  Interpretive
  Guidelines		 A positive titer confirms a diagnosis of Myasthenia gravis. Negative titers can occur in 10 to 20% of cases, therefore, a negative result does not exclude Myasthenia gravis.
  T435 ACTH (Endogeneous Levels)
  Specimen Plasma from LT containing aprotinin
  Method RIA
  Schedule 7 working days
  Indication To differentiate pituitary-dependent from adrenal tumor hyperadrenocorticisim.
  Interpretive
  Guidelines		 Endogenous ACTH concentrations will be increased (> 45 pg/ml) in approximately 90% of dogs with PDH and will be decreased (< 15 pg/ml) in approximately 60% of dogs with adrenal tumor hyperadrenocorticiam. a value between 15 and 45 pg/ml is non-diagnostic.
  Comments Use LT containing aprotinin for sample collection, cerntrifuge immediately and transfer plasma to a plastic vial. (Antech will provide the necessary tubes.) Transport on ice or cold packs. Do not use any plain, uncoated glass tubes or pipettes. Please allow for a minimum of three days to obtain the special container from our supply department.
  T440 ACTH Response Test (Cortisol 2)
  Specimen 1 ml serum or heparinized plasma per tume, label tubes PRE and POST
  Method RIA
  Schedule Daily (M–Sat)
  Indication Investigation of hypoadrenocorticism or hyperadrenocorticism. Monitoring response to Lysodren or Trilostane treatment.
  Special
  Drawing
  Instructions

Canine (using Acthar ACTH gel):

  1. Collect pre-sample; label tube "Pre-ACTH".
  2. Inject 2.2 IU/kg (maximum 40 units) ACTH gel IM.
  3. Collect 2 hr post-sample; label "2 hr Post ACTH".
Canine (using synthetic ACTH)
  1. Collect pre-sample; label tube "Pre-ACTH".
  2. Inject 0.25 mg Cosyntropin (Cortrosyn) IM or IV OR inject 5 ug/kg IV only.
  3. Collect 1 hr post-sample; label "1 hr Post ACTH".
Feline (using Acthar ACTH gel)
  1. Collect pre-sample; label tube "Pre-ACTH".
  2. Inject 2.2 IU/kg ACTH gel IM.
  3. Collect 1 hr and 2 hr post-samples; label accordingly.
Feline (using synthetic ACTH)
  1. Collect pre-sample; label tube "Pre-ACTH".
  2. Inject 0.125 mg Cosyntropin (Cortrosyn) IM or IV.
  3. Collect 1 hr post-sample; label accordingly.
Equine
  1. Draw a resting cortisol. Centrifuge tube, remove the serum and refrigerate.
  2. Inject ACTH gel: 0.5 I.U. per pound given IM or synthetic ACTH get may be used 100 I.U. given IV or IM.
  3. Collect a 4 hr post sample if using ACTH gel.
    Collect a 2 hr post sample if cosyntropin is given IV.
    Collect a 2 & 4 hr post sample if cosyntropin is given IM.
    Centrifuge the tube, remove the serum and refrigerate.
  Comments If animal is on Prednisone, wait 24 hours before doing test. If animal has received Methyl Prednisone acetate, wail 6 weeks before drawing. Dexamethasone will not interfere with cortisol assay, other than supressing the pre value.
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